PhanerochaeteV1, Main, Exploration, bibRecord, 000A88

Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes.

Identifieur interne : 000A88 ( Main/Exploration ); précédent : 000A87; suivant : 000A89

Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes.

Auteurs : Y F Dufrêne [Belgique]

Source :

Biophysical journal [ 0006-3495 ] ; 2000.

RBID : pubmed:10828004

Descripteurs français

KwdFr :
- Adhérence cellulaire (MeSH), Indicateurs et réactifs (MeSH), Microscopie à force atomique (méthodes), Phanerochaete (physiologie), Phanerochaete (ultrastructure), Reproductibilité des résultats (MeSH), Spores fongiques (physiologie), Spores fongiques (ultrastructure).
MESH :
- méthodes : Microscopie à force atomique.
- physiologie : Phanerochaete, Spores fongiques.
- Adhérence cellulaire, Indicateurs et réactifs, Phanerochaete, Reproductibilité des résultats, Spores fongiques.

English descriptors

KwdEn :
- Cell Adhesion (MeSH), Indicators and Reagents (MeSH), Microscopy, Atomic Force (methods), Phanerochaete (physiology), Phanerochaete (ultrastructure), Reproducibility of Results (MeSH), Spores, Fungal (physiology), Spores, Fungal (ultrastructure).
MESH :
- chemical : Indicators and Reagents.
- methods : Microscopy, Atomic Force.
- physiology : Phanerochaete, Spores, Fungal.
- ultrastructure : Phanerochaete, Spores, Fungal.
- Cell Adhesion, Reproducibility of Results.

Abstract

A new method is described for characterizing the physicochemical properties of native microbial cells by using atomic force microscopy (AFM) with chemically functionalized probes. Adhesion forces were measured, under deionized water, between probes and model substrata functionalized with alkanethiol self-assembled monolayers terminated with OH and CH(3) groups. These were found to be 6 +/- 2 nN (n = 1024), 0.9 +/- 0.4 nN, and approximately 0 nN, for CH(3)/CH(3), CH(3)/OH, and OH/OH surfaces, respectively, and were not significantly influenced by changes of ionic strength (0.1 M NaCl versus deionized water). This shows that functionalized probes are very sensitive to changes of surface hydrophobicity. Using OH- and CH(3)-terminated probes, patterns of rodlets, approximately 10 nm in diameter, were visualized, under physiological conditions, at the surface of spores of Phanerochaete chrysosporium. Multiple (1024) force-distance curves recorded over 500 x 500-nm areas at the spore surface, either in deionized water or in 0.1 M NaCl solutions, always showed no adhesion for both OH- and CH(3)-terminated probes. Control experiments indicated that the lack of adhesion is not due to transfer of cellular material onto the probe, but to the hydrophilic nature of the spore surface.

DOI: 10.1016/S0006-3495(00)76864-0
PubMed: 10828004
PubMed Central: PMC1300909

Affiliations:

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Le document en format XML

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<term>Phanerochaete (physiology)</term>
<term>Phanerochaete (ultrastructure)</term>
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<term>Phanerochaete (physiologie)</term>
<term>Phanerochaete (ultrastructure)</term>
<term>Reproductibilité des résultats (MeSH)</term>
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<front><div type="abstract" xml:lang="en">A new method is described for characterizing the physicochemical properties of native microbial cells by using atomic force microscopy (AFM) with chemically functionalized probes. Adhesion forces were measured, under deionized water, between probes and model substrata functionalized with alkanethiol self-assembled monolayers terminated with OH and CH(3) groups. These were found to be 6 +/- 2 nN (n = 1024), 0.9 +/- 0.4 nN, and approximately 0 nN, for CH(3)/CH(3), CH(3)/OH, and OH/OH surfaces, respectively, and were not significantly influenced by changes of ionic strength (0.1 M NaCl versus deionized water). This shows that functionalized probes are very sensitive to changes of surface hydrophobicity. Using OH- and CH(3)-terminated probes, patterns of rodlets, approximately 10 nm in diameter, were visualized, under physiological conditions, at the surface of spores of Phanerochaete chrysosporium. Multiple (1024) force-distance curves recorded over 500 x 500-nm areas at the spore surface, either in deionized water or in 0.1 M NaCl solutions, always showed no adhesion for both OH- and CH(3)-terminated probes. Control experiments indicated that the lack of adhesion is not due to transfer of cellular material onto the probe, but to the hydrophilic nature of the spore surface.</div>
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Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes.

Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki